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@#AIM: To investigate the correlation between erythrocyte immune function and serum erythropoietin(EPO)and plasma endothelin-1(ET-1)in patients with angle-closure glaucoma.<p>METHODS: Totally 30 patients with angle-closure glaucoma(case group)and 30 normal eyes(control group)were selected from our hospital from June 2017 to October 2017. The erythrocyte immune function, serum EPO and plasma ET-1 levels were measured and compared between the two groups.<p>RESULTS: The rosette rate of erythrocyte C3b receptor in the case group was significantly lower than that in the control group(10.81%±2.01% <i>vs</i> 18.06%±3.44%), the rosette rate of erythrocyte immune complex was significantly higher than that in the control group(17.21%±3.49% <i>vs</i> 11.74%±2.14%), the serum EPO concentration was significantly higher than that in the control group(26.10%±5.22mU/mL <i>vs</i> 22.68%±4.06mU/mL), and the plasma ET-1 concentration was significantly higher than that in the control group(70.85%±7.16ng/L <i>vs</i> 58. 43±5.09ng/L). The rosette rate of erythrocyte C3b receptor in patients with angle-closure glaucoma was positively correlated with serum EPO concentration(<i>r</i>=0.271, <i>P</i><0.05), but not with plasma ET-1 concentration.<p>CONCLUSION: There is a positive correlation between erythrocyte immune function and serum EPO concentration in patients with angle-closure glaucoma.
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As the P2X receptor family has become a research hotspot in recent years, the study on P2X7 receptor has also received extensive attention. P2X7 receptor (P2X7R) is a dual-transmembrane cation-channel receptor, which is gated in vivo by adenosine triphosphate (ATP) and can be activated. Activated P2X7 receptor can produce such effects as cationic channel opening, signal pathway activation, inflammatory mediator release and cell apoptosis. It plays an important role in the development of various tumors and inflammatory diseases. Studies have shown that the abnormal expression of P2X7 receptor affects the occurrence and development of breast cancer by activating a series of signaling pathways, which is expected to become a new target for designing anti-breast cancer drugs. Other studies have shown that multiple microRNAs can promote the occurrence and development of breast cancer by regulating the expression of P2X7 receptor gene. In this review, we reviewed the recent research advances of P2X7 receptor and the relationship between breast cancer and its abnormal expression.
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<p><b>OBJECTIVE</b>Osteosarcoma is the most common type of malignant bone tumor in children and adolescents. The role of E3 ligases in tumorigenesis is currently a focus in tumor research. In the present study, we investigated the role of the E3 ligase tripartite motif 21 (TRIM21) in osteosarcoma cell proliferation.</p><p><b>METHODS</b>3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays were used to assess osteosarcoma cell viability. U2-OS cells stably carrying a recombinant lentivirus expressing tetracycline-regulated TRIM21 were screened. Co-immunoprecipitation was coupled with LCMS/MS analysis to identify novel interacting partners of TRIM21. Co-immunoprecipitation and bimolecular fluorescence complementation (BIFC) were performed to validate the interactions between TRIM21 and its novel partner YWHAZ. A TRIM21-ΔRING construct was generated to test the effects of TRIM21 ligase activity on YWHAZ.</p><p><b>RESULTS</b>TRIM21 positively regulated osteosarcoma cell proliferation. Overexpression of TRIM21 enhanced osteosarcoma cell tolerance toward various stresses. YWHAZ protein was identified as a novel interacting partner of TRIM21 and its expression levels were negatively regulated by TRIM21. The RING domain of TRIM21 was required for TRIM21 negative regulation of YWHAZ expression. However, overexpression of YWHAZ did not affect positive regulation of osteosarcoma cell proliferation by TRIM21.</p><p><b>CONCLUSION</b>Our results further clarify the molecular mechanisms underlying the pathogenesis of osteosarcoma.</p>
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Humans , 14-3-3 Proteins , Genetics , Metabolism , Cell Proliferation , Genetics , Osteosarcoma , Genetics , Ribonucleoproteins , Genetics , Metabolism , Tumor Cells, CulturedABSTRACT
·AIM: To investigate the visual recovery factors in patients with complex traumatic and non-traumatic retinal detachment. ·METHODS: According to the history of ocular trauma before admission, 135 patients with complex retinal detachment were divided into traumatic group ( n=66, 66 eyes) and non-traumatic group (n=69, 69 eyes). The results of visual recovery and complications were compared between the two groups. Logistic regression was used to analyze the related factors of visual recovery in the two groups. ·RESULTS: There was no significant difference in the recovery rate between the two groups within 24h after surgery (77. 3% vs 78. 3%, P>0. 05), and there was no significant difference in the total incidence of complications at 3mo after surgery(18. 2% vs 17. 4%, P>0. 05). Multifactorial Logistic regression analysis showed significant correlation between age, injury type, time since retinal detachment, rage of retinal detachment, preoperative vitreous blood,proliferative vitreoretinopathy ( PVR ) and postoperative visual recovery in patients with complex traumatic retinal detachment ( P < 0. 05 ); age, time since retinal detachment, rage of retinal detachment and macular status were significantly associated with visual recovery in patients with complex non-traumatic retinal detachment (P<0. 05). ·CONCLUSION: Age, time since retinal detachment and rage of retinal detachment were significantly associated with traumatic and non-traumatic retinal detachment. The injury type, preoperative vitreous hemorrhage, PVR were significantly correlated with the visual recovery of traumatic retinal detachment patients. The condition of macular was significantly associated with the visual recovery of non-traumatic retinal detachment patients.
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<p><b>OBJECTIVE</b>This study aimed to evaluate the effectiveness of multi-disciplinary treatment approaches in reducing neurological disabilities in premature infants.</p><p><b>METHODS</b>A total of 117 infants who were born premature in our hospital between March 2008 and February 2010 but had no congenital malformations and no severe neonatal complications, were enrolled in this study. They were randomly allocated to a multi-disciplinary treatment group (n=63) and a control group (n=54). While patients in the control group underwent an early conventional treatment, those in the multi-disciplinary treatment group were subjected to regular development monitoring, neurological examination and screening for brain injury, neuro-nutrition and neurodevelopment therapies, and rehabilitation training.</p><p><b>RESULTS</b>The incidence rates of abnormalities in posture, reflex, sleep, muscle tone and EEG were significantly lower in the multi-disciplinary treatment group than in the control froup (P<0.05) at corrected postnatal ages of 6-12 months. At corrected postnatal ages of 6, 12, 18 and 24 months, both mental development index (MDI) and psychomotor development index (PDI) scores were significantly higher in the multi-disciplinary treatment group than in the control group (P<0.05). At corrected postnatal age of 3 years, incidence rates of cerebral palsy, language barrier, abnormal muscle tone and hearing impairment were significantly lower in the multi-disciplinary treatment group than in the control group (P<0.05).</p><p><b>CONCLUSIONS</b>Early multi-disciplinary intervention approaches may significantly improve mental and motor developments and reduce the incidence of cerebral palsy-associated neurological disabilities in premature infants.</p>
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Child, Preschool , Female , Humans , Infant , Infant, Newborn , Male , Cerebral Palsy , Developmental Disabilities , Infant, Premature , Infant, Premature, DiseasesABSTRACT
<p><b>OBJECTIVE</b>To study the effects of environmental enrichment on neuron proliferation, learning and memory ability and motor ability in neonatal rats with hypoxic-ischemic brain damage (HIBD).</p><p><b>METHODS</b>One hundred and eight 7-day-old Sprague-Dawley rats were randomly divided into three groups: sham operation (CON group), HIBD and intervention group. HIBD model was prepared according to the classic Rice-Vannucci method. Environmental enrichment was administered for the rats in the intervention group after HIBD inducement. Behavioral tests (Water maze test, Suspension test and Slope test) were performed and the number of neural cells in the left hippocampus was examined 7, 14 and 28 days after intervention.</p><p><b>RESULTS</b>The pyramid cells in the hippocampus CA1 area in the HIBD group were significantly less than in the CON group at 7, 14 and 28 days (P<0.05). The number of pyramid cells in the hippocampus CA1 area in the intervention group was significantly higher than in the HIBD group (P<0.01) at 7, 14 and 28 days. The hidden platform escape latency period (EL) in the Water maze test was significantly more prolonged and the cross-platform number within 2 minutes was significantly less in the HIBD and the intervention groups than in the CON group at all observed time points (P<0.01). The EL was significantly shorter and the cross-platform number within 2 minutes was significantly higher in the intervention group than in the HIBD group at all observed time points (P<0.01). The maintain time and score in the Suspension test were significantly lower and the time in the Slope test was significantly more prolonged in the HIBD and intervention groups than in the CON group at 7, 14 and 28 days (P<0.01). An increased maintain time and score and a decreased time in the Slope test were found in the intervention group compared with the HIBD group at 14 and 28 days (P<0.01).</p><p><b>CONCLUSIONS</b>Environmental enrichment can improve motor function, learning and memory ability, and promote the repair and proliferation of neurons in neonatal rats with HIBD.</p>